C5a (C5C6) Aptamer Details
ID# 7718
Structure
Chemistry
2'-F-RNA
Target
Human complement C5 component (C5a)
Category
Protein
Affinity (Kd)
100 nM
Length
38
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-fCprGprApfUprGpfCprGprGpfUpfCp fUpfCprApfUprGpfCprGpfUpfCprGp rAprGpfUprGpfUprGprAprGpfUpfUp fUprApfCpfCpfUpfUpfCprGp -3'
Molecular Weight: 12189.15
Extinction Coefficient: 365900
Binding Conditions / Buffer
Binding Buffer: Phosphate-buffered saline with 1 mM MgCl2.
Incubated in the presence or absence of trypsin (between 0.3 and 0.0001 μg/ml)
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
G Biesecker et al.. "Derivation of RNA aptamer inhibitors of human complement C5." Immunopharmacology 42(1999):219-230
Request a Quote
Have your aptamer oligo synthesized by Aptagen.
