C3A WWP1 E3 ligase Aptamer Details
ID# 8188
Structure
Chemistry
DNA
Target
C-lobe, HECT domain, WWP1 E3 ligase
Category
Protein
Affinity (Kd)
1.9 nM
Length
74
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dCpdCpdGpdTpdApdApdTpdApdCpdGpdApdCpdTpdCpdApdCpdTpdApdTpdApdGpdGpdTpdApdTpdGpdTpdGpdApdCpdGpdCpdGpdCpdGpdTpdCpdApdApdTpdCpdGpdCpdTpdGpdTpdCpdCpdTpdApdCpdCpdApdApdGpdCpdTpdTpdTpdGpdCpdApdGpdApdGpdApdGpdGpdApdTpdCpdCpdTpdTp-3'
Molecular Weight: 22662.06
Extinction Coefficient: 700533
Binding Conditions / Buffer
50 mM Tris/0.05% Tween-20/0 → 1.0 M NaCl (pH 7.3)
Binding Temp: 23°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Tucker, W., Kinghorn, A., Fraser, L., Cheung, Y.-W., & Tanner, J. (2018). Selection and Characterization of a DNA Aptamer Specifically Targeting Human HECT Ubiquitin Ligase WWP1. International Journal of Molecular Sciences, 19(3), 763. doi:10.3390/ijms19030763
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