C10 Aptamer Details
ID# 8077
Structure
Chemistry
DNA
Target
Rituximab
Category
Protein
Affinity (Kd)
17.6 nM
Length
40
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
3’-dApdCpdTp dTpdCpdGp dGpdCpdTp dApdGpdTp dTpdApdGp dGpdGpdGp dGpdTpdAp dGpdTpdTp dTpdApdGp dApdTpdCp dGpdTpdCp dTpdCpdTp dApdCpdAp dTp-5’
Molecular Weight: 12348
Extinction Coefficient: 386600
Binding Conditions / Buffer
20 mM Tris pH 7.6, 100 mM NaCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02% v/v Tween 20
Binding Temp: 22°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Kohlberger M, Wildner S, Regl C, Huber CG, Gadermaier G. Rituximab-specific DNA aptamers are able to selectively recognize heat-treated antibodies. PLoS One. 2020 Nov 5;15(11):e0241560. doi: 10.1371/journal.pone.0241560. PMID: 33151990; PMCID: PMC7644011.
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