C-Reactive Protein Monomer Aptamer Details

ID# 7565

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Structure

Chemistry

RNA

Target

Monomeric C-Reactive Protein (CRP)

Sequence (3-char table code)

G A C U/T

5'-rGprCprCprUprGprUprAprAprGprGprUprGprGprUprCprGprGprUprGprUprGprGprCprGprAprGprUprGprUprGprUprUprAprGprGprAprGprAprGprAprUprUprGprCp-3'

Molecular Weight: 14346.65

Extinction Coefficient: 444700

Binding Conditions / Buffer

Binding Buffer: 20 mM HEPES, 140 mM NaCl, 50 mM KCl, 0.1 mM DTT, and 5% glycerol at pH 6.5 Incubated for 20 min.

Binding Temp: RT°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

M S Wang et al. "C-reactive protein (CRP) aptamer binds to monomeric but not pentameric form of CRP." Analytical and Bioanalytical Chemistry, 401(2011): 1309-1318. M S Wang and S M Reed. Direct visualization of electrophoretic mobility shift assays using nanoparticle-aptamer conjugates. Electrophoresis. 33(2012): 348-351.

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