C-Reactive Protein (CRP) Aptamer Details

ID# 7719

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Structure

Chemistry

DNA

Target

C-Reactive Protein (CRP)

Sequence (3-char table code)

G A C U/T

5'-dGpdGpdCp dApdGpdGp dApdApdGp dApdCpdAp dApdAp dCpdApdCp dGpdApdTp dGpdGpdGp dGpdGpdGp dGpdTpdAp dTpdGpdAp dTpdTpdTp dGpdApdTp dGpdTpdGp dGpdTpdTp dGpdTpdTp dGpdCpdAp dTpdGpdAp dTpdCpdGp dTpdGpdGp dTpdCpdTp dGpdTpdGp dGpdTpdGp dCpdTpdGp dTp-3'

Molecular Weight: 22571.71

Extinction Coefficient: 704600

Binding Conditions / Buffer

DNA and CRP-conjugated magnetic beads mixed and incubated utilizing a membrane-type micropump/micromixer (for about 5min). Dissociation constant determined using a SPR technique. SPR Buffer: 10 mM Tris buffer with 50 mM NaCl and 2 mM CaCl2.

Binding Temp: 25°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

C-J Huang et al. "Integrated microfluidic system for rapid screening of CRP aptamers utilizing systematic evolution of ligands by exponential enrichment (SELEX)." Biosens. Bioelectron. 25(2010):1761-1766.

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