C-Reactive Protein Aptamer Details
ID# 7819
Structure
Chemistry
RNA
Target
C-Reactive Protein (CRP)
Category
Protein
Affinity (Kd)
125 nM
Length
44
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprCprCprUprGprUprAprAprGprGprUprGprGprUprCprGprGprUprGprUprGprGprCprGprAprGprUprGprUprGprUprUprAprGprGprAprGprAprGprAprUprUprGprCp -3'
Molecular Weight: 14346.65
Extinction Coefficient: 444700
Binding Conditions / Buffer
SPR Binding Buffer: HEPES at pH 6.5 with 0.005% Tween 20
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
A Bini, M Mascini, et al. Development of an optical RNA-based aptasensor for C-reactive protein. Anal. Bioanal. Chem. 390(2008): 1077-1088
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