Bvd4 Aptamer Details
ID# 9631
Structure
Chemistry
DNA
Target
Biliverdin
Category
Small Organic
Affinity (Kd)
6.4 nM
Length
36
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdGpdTpdGpdTpdGpdGpdApdApdCpdApdCpdGpdApdApdCpdGpdTpdApdGpdTpdGpdCpdGpdApdApdTpdApdCpdTpdTpdTpdCpdGpdAp-3'
Molecular Weight: 11189.3 g/mole
Extinction Coefficient: 360900
Binding Conditions / Buffer
Selection buffer - 200 mM NaCl, 10 mM phosphate, 2 mM MgCl2, pH 7.6. The randomized aptamer library was annealed with the biotinylated capture strand in selection buffer. Streptavidin-agarose resin was loaded, and biotin-ssDNA complex was added into it. Bilirubin was loaded onto the library and the eluted ssDNA was obtained through gravity flow.
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Yachen Xie, Yunus A. Kaiyum, Lide Gu, Yibo Liu, Philip E. Johnson, and Juewen Liu
ACS Chemical Biology 2025 20 (8), 2028-2037
DOI: 10.1021/acschembio.5c00438
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