Bovine Thrombin (T7 05 RNA) Aptamer Details

ID# 7472

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Structure

Chemistry

RNA

Target

Bovine Thrombin

Sequence (3-char table code)

G A C U/T

5'-rGprCprAprAprUprGprGprUprAprCprGprGprUprAprCprUprUprCprCprUprUprUprGprGprAprAprGprAprUprAprGprCprUprGprGprAprGprAprAprCprUprAprAprCprCprAprAprAprAprGprUprGprCprAprCprGprCprUprAprCprUprUprUprGprCprUprAprAp-3'

Molecular Weight: 21852.22

Extinction Coefficient: 684600

Binding Conditions / Buffer

SHMCK+ buffer (20 mM Hepes (pH 7.35), 120 mM NaCl, 5 mM KCl, 1 mM CaCl2, 1 mM MgCl2 and 0.1% geletin) containing 0.05% Tween 20. Binding Buffer (SHMCK+ buffer containing 0.05% Tween 20)

Binding Temp: 37°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

X Liu, et al. "RNA aptamers speci?c for bovine thrombin." Journal of Molecular Recognition, 16 (2003): 23-27. X Liu, et al. "Screening of functional antidotes of RNA aptamers against bovine thrombin." FEBS Lett. 562(2004): 125-128

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