Botulinum neurotoxin type A (BoNT A) Aptamer Aptamer Details

ID# 7952

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Structure

Chemistry

DNA

Target

BoNT A Light Chain (BoNT/A-rLc)

Sequence (3-char table code)

G A C U/T

5'-dCpdTpdTpdGpdApdGpdTpdGpdTpdCpdApdTpdGpdGpdApdCpdGpdTpdTpdCpdCpdGpdGpdTpdCpdTpdTpdGpdGpdGpdCpdGpdGpdGpdApdTpdApdTpdTpdTpdGpdTpdTpdTpdGpdTpdTpdTpdTpdCpdTpdGpdCpdCpdTpdApdTpdGpdTpdTp-3'

Molecular Weight: 18544.08

Extinction Coefficient: 547900

Binding Conditions / Buffer

1X Binding Buffer: 20 mM Hepes, 150 mM NaCl, 2 mM KCl, 2 mM MgCl2, 2mM CaCl2 (pH 7.4)

Binding Temp: 23°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

(1) Bogomolova, A., Aldissi, M. ƒ??Real-time and label-free analyte detection in a flow-through mode using immobilized fluorescent aptamer/quantum dots molecular switches.ƒ? Biosensors and Bioelecgtronics, 66 (2015): 290-296. (2) Lou, X., et al. ƒ??Micromagnetic selection of aptamers in microfluidic channels.ƒ? PNAS, 106, 9 (2009): 2989-2994.

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