Bisphenol A(#3) Aptamer Details
ID# 7578
Structure
Chemistry
DNA
Target
Bisphenol A(BPA)
Category
Small Organic
Affinity (Kd)
8.3 nM
Length
109
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdGpdCpdCpdGpdTpdTpdCpdGpdApdApdCpdApdCpdGpdApdGpdCpdApdTpdGpdCpdCpdGpdGpdTpdGpdGpdGpdTpdGpdGpdTpdCpdApdGpdGpdTpdGpdGpdGpdApdTpdApdGpdCpdGpdTpdTpdCpdCpdGpdCpdGpdTpdApdTpdGpdGpdCpdCpdCpdApdGpdCpdGpdCpdApdTpdCpdApdCpdGpdGpdGpdTpdTpdCpdGpdCpdApdCpdCpdApdGpdGpdApdCpdApdGpdTpdApdCpdTpdCpdApdGpdGpdTpdCpdApdTpdCpdCpdTpdApdGpdGp-3'
Molecular Weight: 33800.83
Extinction Coefficient: 1036700
Binding Conditions / Buffer
Binding buffer: 25 mM Tris-HCL, 100 mM NaCl, 25 mM KCl, 10 mM MgCl2, and 5.0% DMSO (pH 8.0)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Jo et al. "Development of Single-Stranded DNA Aptamers for Specific Bisphenol A Detection." Oligonucleotides, 21(2011): 85-91.
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