Biotin Aptamer Details
ID# 7553
Structure
Chemistry
RNA
Target
Biotin
Category
Small Organic
Affinity (Kd)
5.7 µM
Length
110
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprAprAprCprAprCprUprAprUprCprCprGprAprUprGprGprCprAprCprCprGprAprCprCprAprUprAprGprGprCprUprCprGprGprGprUprUprGprCprCprAprGprAprGprGprUprUprCprCprAprCprAprCprUprUprUprCprAprUprCprGprAprAprAprAprGprCprCprUprAprUprGprCprUprAprGprGprCprAprAprUprGprAprCprAprUprGprGprAprCprUprCprCprUprUprGprGprUprCprAprUprUprAprGprGprAprUprCprGp-3'
Molecular Weight: 35326.27
Extinction Coefficient: 1080100
Binding Conditions / Buffer
Selection Buffer (0.1 M KCl, 5 mM MgCl2, and 10 mM Na-HEPES (pH 7.4))
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Wilson et al. "Functional Requirements for Specific Ligand Recognition by a Biotin-Binding RNA Pseudoknot." Journal of Biochemistry, 37(1998): 14410-14419.
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