Beta 2-Integrin (D20) Aptamer Details
ID# 7543
Structure
Chemistry
RNA
Target
Beta 2-Integrin
Category
Protein
Affinity (Kd)
500 nM
Length
73
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprCprGprCprUprAprAprGprUprCprCprUprCprGprCprUprCprAprUprGprCprGprCprGprUprCprCprCprAprUprGprGprGprGprUprAprUprAprGprAprGprGprGprGprUprCprGprAprAprGprUprGprGprAprCprGprCprGprCprGprAprCprUprCprGprGprAprUprCprCprUp-3'
Molecular Weight: 23599.21
Extinction Coefficient: 700300
Binding Conditions / Buffer
Binding Buffer (4.3 mM K2HPO4, 1.4 mM NaH2PO4, 150 mM NaCl, 1.0 mM MgCl2, 0.1 uM CaCl2)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Blind, M., et al. "Cytoplasmic RNA modulators of an inside-out signaltransduction cascade." Proceedings of National Academy of Sciences of the United States of America, 96(1999):3606ƒ??3610.
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