BC1 Aptamer Details
ID# 8099
Structure
Chemistry
DNA
Target
Osteocalcin (OC)
Category
Protein
Affinity (Kd)
69.34 nM
Length
59
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5’-dApdTpdGpdApdCpdGpdGpdGpdGpdGpdTpdCpdTpdApdGpdGpdCpdApdApdGpdTpdApdApdTpdApdApdCpdGpdGpdGpdGpdGpdCpdApdApdGpdCpdTpdTpdTpdTpdCpdTpdApdTpdCpdTpdCpdGpdTpdTpdCpdTpdApdGpdGpdGpdTpdAp-3’
Molecular Weight: 18336.9
Extinction Coefficient: 579500
Binding Conditions / Buffer
2 mM MgCl2, 50 mM Tris (pH 7.5) and 150 mM NaCl in distilled water
Binding Temp: 22°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Chinnappan R, Zaghloul NS, AlZabn R, Malkawi A, Abdel Rahman A, Abu-Salah KM, Zourob M. Aptamer selection and aptasensor construction for bone density biomarkers. Talanta. 2021 Mar 1;224:121818. doi: 10.1016/j.talanta.2020.121818. Epub 2020 Oct 29. PMID: 33379043.
Request a Quote
Have your aptamer oligo synthesized by Aptagen.
