BC 007 – Targeting Viral Proteins Essential for SARS-CoV-2 Replication Aptamer Details

ID# 9134

Structure

Chemistry

DNA

Target

Receptor-binding domain (RBD) of the SARS-CoV-2 spike protein and RNA-dependent RNA polymerase (RdRp). As well as harmful autoantibodies that target G-protein-coupled receptors (GPCR-AABs).

Sequence (3-char table code)

G A C U/T

5'-dGpdGpdTpdTpdGpdGpdTpdGpdTpdGpdGpdTpdTpdGpdGp-3'

Molecular Weight: 4726.1 g/mole

Extinction Coefficient: 143300

Binding Conditions / Buffer

Nuclear Magnetic Resonance Spectroscopy (NMR), High Sensitivity Isothermal Titration Calorimetry (ITC), Circular Dichroism Spectroscopy (CD) Buffer: NaCl/sodium phosphate buffer

Binding Temp: 25°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Weisshoff, H., Krylova, O., Nikolenko, H., Düngen, H. D., Dallmann, A., Becker, S., Göttel, P., Müller, J., & Haberland, A. (2020). Aptamer BC 007 - Efficient binder of spreading-crucial SARS-CoV-2 proteins. Heliyon, 6(11), e05421. https://doi.org/10.1016/j.heliyon.2020.e05421 Werner, S., Wallukat, G., Becker, N. P., Wenzel, K., Müller, J., Schimke, I., & Wess, G. (2020). The aptamer BC 007 for treatment of dilated cardiomyopathy: evaluation in Doberman Pinschers of efficacy and outcomes. ESC heart failure, 7(3), 844–855. https://doi.org/10.1002/ehf2.12628

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