BB16-11 f Aptamer Details
ID# 8210
Structure
Chemistry
DNA
Target
Bifidobacterium Breve
Category
Cells
Affinity (Kd)
18.66 nM
Length
29
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dCpdTpdCpdCpdCpdApdGpdGpdCpdCpdGpdTpdTpdGpdGpdGpdGpdCpdGpdTpdTpdGpdCpdCpdTpdGpdCpdGpdTp-3'
Molecular Weight: 8972.73
Extinction Coefficient:
Binding Conditions / Buffer
50 mM Tris–HCl (pH 7.4), 100 mM NaCl, 5 mM KCl and 1 mM MgCl2.
Binding Temp: 21°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Hu, L., Wang, L., Lu, W., Zhai, Q., Fan, D., Liu, X., … Chen, W. (2017). Selection, identification and application of DNA aptamers for the detection of Bifidobacterium breve. RSC Advances, 7(19), 11672–11679.
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