Basic Fibroblast Growth Factor (26A-t) Aptamer Details
ID# 7540
Structure
Chemistry
RNA
Target
Basic Fibroblast Growth Factor (bFGF)
Category
Protein
Affinity (Kd)
190 pM
Length
34
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprUprGprAprAprGprGprCprAprAprCprGprUprAprUprAprGprGprCprAprAprGprCprAprCprAprCprUprUprCprAprCprCp-3'
Molecular Weight: 10943.71
Extinction Coefficient: 340500
Binding Conditions / Buffer
Selection Buffer (1X PBS = 10.1 mM Na2HPO4/1.8 mMKH2PO4/137 mM NaCl/2.7 mM KCl, pH 7.4)
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Jellinek, D., et al. "High-affinity RNA ligands to basic fibroblast growth factor inhibit receptor binding." Proceedings of the National Academy of Sciences of the United States of America, 90(1993): 11227ƒ??11231.
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