B1-4 Aptamer Details
ID# 7903
Structure
Chemistry
DNA
Target
IFN-Gamma
Category
Protein
Affinity (Kd)
74.5 nM
Length
59
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dCpdCpdGpdCpdCpdCpdApdApdApdTpdCpdCpdCpdTpdApdApdGpdApdGpdApdApdGpdApdCpdTpdGpdTpdApdApdTpdGpdApdCpdApdTpdCpdApdApdApdCpdCpdApdGpdApdCpdApdCpdApdCpdTpdApdCpdApdCpdApdCpdGpdCpdAp-3'
Molecular Weight: 18001.76
Extinction Coefficient: 585100
Binding Conditions / Buffer
200 uL (PBS), 0.05 pM Aptamer
Binding Temp: 24°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Cao B, Hu Y, Duan J, Ma J, Xu D, et al. (2014) Selection of a Novel DNA Aptamer for Assay of Intracellular Interferon-Gamma. PLoS ONE 9(5): e98214. doi:10.1371/journal.pone.0098214
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