B01 Aptamer Details
ID# 8128
Structure
Chemistry
DNA
Target
β2-Microglobulin (B2M)
Category
Protein
Affinity (Kd)
52.9 nM
Length
80
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5′-dCpdTpdTpdCpdTpdGpdCpdCpdCpdGpdCpdCpdTpdCpdCpdTpdTpdCpdCpdTpdCpdGpdTpdGpdApdCpdGpdCpdCpdGpdTpdGpdCpdApdGpdTpdTpdApdCpdCpdGpdApdTpdApdCpdGpdApdGpdCpdCpdTpdCpdGpdTpdTpdTpdTpdGpdGpdGpdApdGpdApdCpdGpdApdGpdApdTpdApdGpdGpdCpdGpdGpdApdCpdApdCpdTp-3′
Molecular Weight: 24550.8
Extinction Coefficient: 732200
Binding Conditions / Buffer
Ammonium bicarbonate buffer (50 mM, pH 8.5) containing 500 mM NaCl.
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Ma, Y.; Li, W.; Xing, R.; Li, P.; Liu, Z. Epitope-Imprinted Magnetic Nanoparticles as a General Platform for Efficient In Vitro Evolution of Protein-Binding Aptamers. ACS Sens. 2020 Jul 7; 5(8): 2537-2544. DOI: 10.1012/acssensors.0c00846
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