Azo-1 Aptamer Details
ID# 8135
Structure
Chemistry
DNA
Target
Human Thrombin
Category
Protein
Affinity (Kd)
Not given. nM
Length
70
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5’-dTpdCpdGpdCpdCpdTpdTpdGpdCpdCpdGpdGpdApdTpdCpdGpdCpdApdGpdApdGprUpdApdApdGpdGpdCpdApdGpdCprUprUpdApdCpdApdApdApdCpdAprUprUpdApdApdGpdGpdCpdApdCpdApdGprUpdGpdGprUpdCpdCpdGprUpdGpdApdGpdCpdCprUpdGpdApdCpdApdCpdCp-3’
Molecular Weight: 21442.7
Extinction Coefficient: 658400
Binding Conditions / Buffer
20 mM Tris-HCl (pH 7.4), 1 mM MgCl2, 10 mM NaCl, and 0.05%Tween-20.
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Morihiro, K.; Hasegawa, O.; Kasahara, Y.; Mori, S.; Kasai, T.; Kuwahara, M.; Obika, S. Azobenzene-modified DNA aptamers evolved by capillary electrophoresis (CE)-SELEX method. Bioorg. Med. Chem. Lett. 2021 Jan 1; 31: 127607. DOI: 10.1016/j.bmcl.2020.127607.
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