Avian Myeloblastosis Virus (a.1.1) Aptamer Details
ID# 7574
Structure
Chemistry
RNA
Target
Avian Myeloblastosis Virus (AMV) RT
Category
Protein
Affinity (Kd)
0.5 nM
Length
43
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rCprGprUprCprCprCprGprUprGprCprGprCprAprAprAprAprGprUprUprCprUprUprAprGprCprGprCprUprAprGprCprAprGprUprCprCprUprAprGprUprUprGprCp-3'
Molecular Weight: 13704.24
Extinction Coefficient: 408400
Binding Conditions / Buffer
Binding Buffer (50 mM Tris-HC1 (pH 7.7), 200 mM potassium acetate, and 10 mM DTT)
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Chen and Gold. "Selection of High-Affinity RNA Ligands to Reverse Transcriptase: Inhibition of cDNA Synthesis and RNase H Activity." Journal of Biochemistry, 33(1994): 8746-8756.
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