Aptagen

Atrazine R12.23 Aptamer Details

ID# 7936
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Structure
Atrazine R12.23 structure
Chemistry
DNA
Target
Atrazine
Category
Small Organic
Affinity (Kd)
0.62 nM
Length
79
Aptazyme
No

Sequence (3-char table code)

G A C U/T
dTpdGpdTpdApdCpdCpdGpdTpdCpdTpdGpdApdGpdCpdGpdApdTpdTpdCpdGpdTpdApdCpdGpdApdApdCpdGpdGpdCpdTpdTpdTpdGpdTpdApdCpdTpdGpdTpdTpdTpdGpdCpdApdCpdTpdGpdGpdCpdGpdGpdApdTpdTpdTpdApdGpdCpdCpdApdGpdTpdCpdApdGpdTpdGpdTpdTpdApdApdGpdGpdApdGpdTpdGpdCp
Molecular Weight: 24464.91
Extinction Coefficient: 749900

Binding Conditions / Buffer

500 µL of buffer consisting of 100 mM sodium chloride, 20 mM Tris-HCl, and 2 mM magnesium chloride
Binding Temp: 24°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Williams, R.; Crihfield, C.; Gattu, S.; Holland, L.; Sooter, L. In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine. International Journal of Molecular Sciences, 2014, 15, 14332-14347. ISSN 1422-0067 doi: 10.3990/ijms150814332
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