Arsenic(III) (Ars-3) Aptamer Details
ID# 7825
Structure
Chemistry
DNA
Target
Arsenic(III)
Category
Small Organic
Affinity (Kd)
4.95 nM
Length
100
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdGpdTpdApdApdTpdApdCpdGpdApdCpdTpdCpdApdCpdTpdApdTpdApdGpdGpdGpdApdGpdApdTpdApdCpdCpdApdGpdCpdTpdTpdApdTpdTpdCpdApdApdTpdTpdTpdTpdApdCpdApdGpdApdApdCpdApdApdCpdCpdApdApdCpdGpdTpdCpdGpdCpdTpdCpdCpdGpdGpdGpdTpdApdCpdTpdTpdCpdTpdTpdCpdApdTpdCpdGpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTp-3'
Molecular Weight: 30767.08
Extinction Coefficient: 981200
Binding Conditions / Buffer
Selection buffer: 150 mM NaCl, 50mM HEPES, 0.5 mM MgCl2, 0.5 mM CaCl2
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Kim, et al. "Arsenic Removal from Vietnamese Groundwater Using the Arsenic-Binding DNA Aptamer." Environ. Sci. Technol., 43 (2009): 9335-9340.
Wu et al. "Ultrasensitive Aptamer Biosensor for Arsenic(III) Detection in Aqueous Solution Based on Surfactant-Induced Aggregation of Gold Particles." Analyst, 137 (2012): 4171-4178.
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