AptHis-C Aptamer Details
ID# 8132
Structure
Chemistry
DNA
Target
Hexahistidine
Category
Small Organic
Affinity (Kd)
20.8 nM
Length
39
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5′-dTpdGpdGpdCpdApdApdGpdApdGpdGpdGpdTpdGpdTpdGpdCpdTpdTpdApdApdGpdGpdTpdGpdGpdApdCpdApdCpdGpdGpdTpdGpdGpdCpdTpdTpdApdGp-3′
Molecular Weight: 12223.9
Extinction Coefficient: 384000
Binding Conditions / Buffer
1% BSA, 0.1% Tween-20, 0.2 mg/mL of tRNA, DPBS, pH 7.4.
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Wu, S.; Liu, Y.; Sun, H.; Zhong, M.; Dai, B.; Pan, B.; Shen, Z. An ssDNA aptamer specific for detection and purification of hexahistidine-tagged proteins. Anal. Biochem. 2020 Oct 15; 607: 113893. DOI: 10.1016/j.ab.2020.113893.
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