Aptamer ID 2 Aptamer Details
ID# 7897
Structure
Chemistry
DNA
Target
PDGF-BB
Category
Protein
Affinity (Kd)
2.5 nM
Length
80
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
dTpdCpdCpdCpdApdCpdGpdCpdApdTpdTpdCpdTpdCpdCpdApdCpdApdTpdCpdGpdApdTpdApdCpdTpdGpdApdGpdCpdApdTpdCpdGpdTpdApdCpdApdTpdGpdApdTpdCpdCpdCpdGpdCpdApdApdCpdGpdGpdGpdCpdApdGpdTpdApdTpdTpdCpdCpdTpdTpdTpdCpdTpdGpdTpdCpdCpdTpdTpdCpdCpdGpdTpdCpdApdCp
Molecular Weight: 24307.75
Extinction Coefficient: 728600
Binding Conditions / Buffer
0.1 mM Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl, and 1 mM MgCl2 (PBSM), pH 7.4
Binding Temp: 23°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Cho et al. "Quantitative selection of DNA aptamers through microfluidic selection and high-throughput sequencing." PNAS 2010, 1-6.
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