Aptamer Beacon Aptamer Details
ID# 8174
Structure
Chemistry
DNA
Target
adenosine
Category
Protein
Affinity (Kd)
6.0 nM
Length
27
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdCpdCpdTpdGpdGpdGpdGpdGpdApdGpdTpdApdTpdTpdGpdCpdGpdGpdApdGpdGpdApdApdGpdGpdTp-3'
Molecular Weight: 8459.31
Extinction Coefficient: 279432
Binding Conditions / Buffer
150 mmol L−1 NaCl, 1 mmol L−1 MgCl2, 5 mmol L−1 KCl and Tris
buffer of 20 mmol L−1 (pH 7.4)
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Zhang JQ, Wang YS, Xue JH, He Y, Yang HX, Liang J, Shi LF, Xiao XL. A gold nanoparticles-modified aptamer beacon for urinary adenosine detection based on structure-switching/fluorescence-"turning on" mechanism. J Pharm Biomed Anal. 2012 Nov;70:362-8. doi: 10.1016/j.jpba.2012.05.032. Epub 2012 Jun 1. PMID: 22717140.
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