Aptamer Apt5 against Spike (S) Protein of SARS-CoV-2 Aptamer Details

ID# 9640

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Structure

Chemistry

DNA

Target

Spike Protein (S -Protein) of SARS-CoV-2

Sequence (3-char table code)

G A C U/T

5'-dApdTpdCpdCpdApdGpdApdGpdTpdGpdApdCpdGpdCpdApdGpdCpdApdGpdGpdApdCpdTpdGpdCpdTpdApdGpdGpdApdTpdTpdGpdCpdGpdApdApdGpdCpdTpdGpdApdGpdGpdApdGpdCpdTpdCpdCpdCpdCpdCpdGpdCpdCpdTpdTpdGpdGpdApdCpdApdCpdGpdGpdTpdGpdGpdCpdTpdTpdApdGpdTp-3'

Molecular Weight: 23526.2 g/mole

Extinction Coefficient: 722500

Binding Conditions / Buffer

Phosphate-buffered saline (PBS; 10 mM phosphate buffer, 137 mM NaCl, 2.7 mM KCl, pH 7.4), Aptamer Binding Buffer was PBS containing 0.55 mM MgCl2.

Binding Temp: 25°C

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Svobodova, M., Skouridou, V., Jauset-Rubio, M., Viéitez, I., Fernández-Villar, A., Cabrera Alvargonzalez, J. J., Poveda, E., Bofill, C. B., Sans, T., Bashammakh, A., Alyoubi, A. O., & O'Sullivan, C. K. (2021). Aptamer Sandwich Assay for the Detection of SARS-CoV-2 Spike Protein Antigen. ACS omega, 6(51), 35657–35666. https://doi.org/10.1021/acsomega.1c05521.

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