Apt22 Aptamer Details
ID# 8212
Structure
Chemistry
DNA
Target
Salmonella Paratyphi A
Category
Cells
Affinity (Kd)
47 nM
Length
41
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdTpdGpdGpdApdCpdGpdApdApdTpdApdTpdCpdGpdTpdCpdTpdCpdCpdCpdApdGpdTpdGpdApdApdTpdTpdCpdApdGpdTpdCpdGpdGpdApdCpdApdGpdCpdGp-3'
Molecular Weight: 12713.23
Extinction Coefficient:
Binding Conditions / Buffer
50 mM Tris-HCl (pH 7.4), 100 mM NaCl, 5 mM KCl, 1 mM MgCl2, and 0.1% NaN3
Binding Temp: 37°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Yang, M., Peng, Z., Ning, Y., Chen, Y., Zhou, Q., Deng, L., 2013. Highly specific and cost-efficient detection of salmonella paratyphi a combining aptamers with single-walled carbon nanotubes. Sensors 13, 6865–6881.
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