Apt08rc Aptamer Details

ID# 8207

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Structure

Chemistry

DNA

Target

Cathepsin B

Sequence (3-char table code)

G A C U/T

5'-dGpdGpdGpdCpdApdGpdGpdCpdApdApdCpdCpdTpdCpdCpdCpdApdCpdTpdApdTpdTpdGpdApdApdGpdApdCpdApdCpdTpdApdCpdCpdApdCpdCpdApdCpdCpdApdCpdTpdGpdCpdApdCpdApdCpdAp-3'

Molecular Weight: 16500

Extinction Coefficient:

Binding Conditions / Buffer

Binding Buffer: PBS 1x (pH = 7.4) with 1.0 mM of CaCl2*2H2O and 0.5 mM MgCl2*6H2O Washing Buffer: Tween 20 at 0.05% v/v added to binding buffer, pH = 8.

Binding Temp: 20°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Pereira, A. C., Pina, A. F., Sousa, D., Ferreira, D., Santos-Pereira, C., Rodrigues, J. L., Melo, L. D. R., Sales, G., Sousa, S. F., & Rodrigues, L. R. (2022). Identification of novel aptamers targeting cathepsin B-overexpressing prostate cancer cells. Molecular Systems Design & Engineering, 7(6), 637–650. https://doi.org/10.1039/d2me00022a

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