Apt01 Aptamer Details
ID# 8200
Structure
Chemistry
DNA
Target
CatB
Category
Protein
Affinity (Kd)
9.0 nM
Length
51
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dTpdGpdGpdGpdGpdGpdGpdCpdApdApdGpdCpdApdApdTpdTpdGpdGpdTpdGpdTpdGpdTpdGpdGpdTpdGpdTpdApdGpdCpdApdTpdCpdCpdCpdCpdCpdTpdGpdTpdApdCpdCpdGpdCpdGpdCpdGpdGpdGp-3'
Molecular Weight: 15810
Extinction Coefficient: 482049
Binding Conditions / Buffer
The binding buffer (BB)
was prepared by supplementing phosphate buffered saline
(PBS) 1× pH 7.4 with 1 mM of CaCl2·2H2O (Panreac) and 0.5
mM of MgCl2·6H2O (VWR).
Binding Temp: 20°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Periera, A., Pina, A., Sousa, D., Ferriera, D., Periera, C., Rodrigues, J., Melo, L., Sales, G., Sousa, S., & Rodrigues, L. (2022). Identification of Novel Aptamers Targeting Cathepsin B-Overexpressing Prostate Cancer Cells, 1–14. https://doi.org/10.1039/d2me00022a
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