Apt 15-25 Aptamer Details
ID# 9506
Structure
Chemistry
DNA
Target
Equine Herpesvirus Type 1 (EHV-1)
Category
Small Organic
Affinity (Kd)
G-quadruplex–forming oligonucleotides were prepared at a final concentration of 7.5 μM in 25 mM Tris–HCl buffer (pH 7.0) containing varying NaCl concentrations. The mixtures were statically incubated to promote proper folding. Crystal violet (CV) was then added to a final concentration of 15 μM, and the mixtures were incubated at 30 °C for 1 h.
Length
30
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdCpdCpdTpdApdGpdGpdGpdGpdTpdApdCpdGpdGpdGpdGpdCpdCpdApdGpdGpdGpdGpdCpdCpdTpdGpdGpdGpdGp-3'
Molecular Weight: 9395.1 g/mole
Extinction Coefficient: 289400
Binding Conditions / Buffer
30°C
Binding Temp: 10-min heat treatment at 95 °C
Refolding Protocol
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Nahid Davoudi, Mandana Behbahani, Hassan Mohabatkar, Ghasem Dini, Mehran Bakhshesh. A novel G-quadruplex aptamer with high affinity for the specific detection of equine herpesvirus type 1: Comprehensive biophysical and analytical characterization,
Talanta, Volume 297, Part B. 2026. 128713. ISSN 0039-9140. https://doi.org/10.1016/j.talanta.2025.128713.
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