APT-1 Aptamer Details
ID# 8204
Structure
Chemistry
DNA
Target
Platelets
Category
Cells
Affinity (Kd)
109.28 nM
Length
79
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdCpdCpdTpdGpdTpdTpdGpdTpdGpdApdGpdCpdCpdTpdCpdCpdTpdApdApdCpdGpdApdCpdApdCpdApdCpdCpdApdApdApdCpdTpdGpdGpdApdGpdCpdCpdApdTpdGpdCpdApdGpdGpdTpdApdGpdGpdApdApdCpdGpdGpdGpdTpdApdCpdApdTpdGpdCpdTpdTpdApdTpdTpdCpdTpdTpdGpdTpdCpdTpdCpdCpdCp-3'
Molecular Weight: 24340.72
Extinction Coefficient:
Binding Conditions / Buffer
Binding buffer included:
NaCl (129 mM)
KCl (2.8 mM)
NaHCO3 (8.9 mM)
KH2PO4 (0.8 mM)
MgCl2 (0.8 mM)
CaCl2 (1 mM)
Glucose (5.6 mM)
HEPES (10 mM)
BSA (0.35%)
prepared in deionized water
Binding Temp: 18°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Alemi, F, et al. The identification of single strand DNA aptamers which specifically bind to platelets using cell SELEX technique. Iranian Journal of Veterinary Science and Technology, 13 (2021): 68-83. doi: https://dx.doi.org/10.22067/ijvst.2021.72518.1079
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