Aptagen

API7-4 Aptamer Details

ID# 9205
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Structure
API7-4 structure
Chemistry
DNA
Target
Small Organic
Category
< 10 nM (reported value)
Affinity (Kd)
Binding buffer: PBST (Phosphate Buffered Saline with Tween 20) + 5 mM Mg²⁺
Length
39
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-dGpdGpdApdApdCpdApdCpdGpdApdCpdCpdGpdTpdTpdGpdTpdGpdGpdCpdCpdApdTpdApdCpdApdGpdTpdApdCpdApdGpdCpdApdTpdGpdTpdGpdGpdGpdCp-3'
Molecular Weight:
Extinction Coefficient:

Binding Conditions / Buffer

37°C
Binding Temp: Not explicitly mentioned in the paper. However, the aptamers were likely refolded by heating and cooling, a common practice in aptamer studies, though the specific program is not detailed.

Refolding Protocol

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Prasad BVV, Hardy ME, Dokland T, Bella J, Rossmann MG, Estes MK. X-ray crystallographic structure of the Norwalk virus capsid. Science. 1999;286(5438):287–290. doi:10.1126/science.286.5438.287
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