AP32-4 Aptamer Details
ID# 8118
Structure
Chemistry
DNA
Target
Metronidazole
Category
Small Organic
Affinity (Kd)
77.22 ± 11.27 nM
Length
16
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dCpdTpdGpdTpdTpdTpdGpdGpdTpdApdGpdGpdCpdApdGp -3'
Molecular Weight: 4639
Extinction Coefficient: 143000
Binding Conditions / Buffer
200mL binding buffer: 50 mM Tris-HCl containing 5 mM KCl, 100 mM NaCl and 1 mMMgCl2, pH 7.4
Binding Temp: 22°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Wei, Hao et al. “Screening and application of a truncated aptamer for high-sensitive fluorescent detection of metronidazole.” Analytica chimica acta vol. 1128 (2020): 203-210. doi:10.1016/j.aca.2020.07.003
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