AP273 (Alpha-fetoprotein) Aptamer Details
ID# 8229
Structure
Chemistry
DNA
Target
Alpha Fetoprotein (AFP)
Category
Protein
Affinity (Kd)
17.4 nM
Length
75
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdTpdGpdApdCpdGpdCpdTpdCpdCpdTpdApdApdCpdGpdCpdTpdGpdApdCpdTpdCpdApdGpdGpdTpdGpdCpdApdGpdTpdTpdCpdTpdCpdGpdApdCpdTpdCpdGpdGpdTpdCpdTpdTpdGpdApdTpdGpdTpdGpdGpdGpdTpdCpdCpdTpdGpdTpdCpdCpdGpdTpdCpdCpdGpdApdApdCpdCpdApdApdTpdCp-3'
Molecular Weight:
Extinction Coefficient:
Binding Conditions / Buffer
The ssDNA library was dissolved in the CE Buffer (30 mM NaH2PO4) at a pH of 7.5. See source paper for more information.
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Screening and Identifying a Novel ssDNA Aptamer against Alpha-fetoprotein Using CE-SELEX
Lili. Dong, et. al. (2015)
https://www.nature.com/articles/srep15552
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