Ap 25 Aptamer Details
ID# 9375
Structure
Chemistry
DNA
Target
Omicron SARS-COV-2 Nucleocapsid
Category
Small Organic
Affinity (Kd)
Aptamers (200 nM concentration in folding buffer) are heated to 95 degrees Celsius for 5 minutes and placed on ice.
Length
73
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dCpdGpdApdGpdGpdCpdTpdCpdTpdCpdGpdGpdGpdApdCpdGpdApdCpdApdTpdCpdTpdTpdCpdApdGpdCpdGpdApdTpdTpdCpdTpdTpdApdApdApdCpdCpdTpdTpdApdGpdApdGpdTpdApdTpdGpdTpdCpdCpdCpdGpdCpdCpdTpdTpdTpdApdGpdGpdApdTpdTpdTpdApdCpdApdGp-3'
Molecular Weight: 22428.5 g/mole
Extinction Coefficient: 689900
Binding Conditions / Buffer
37°C
Binding Temp: Nucleocapsid proteins are incubated with 100 ul of aptamers at a 200 nM concentration for 2 hours and washed with 300 ul of 1x ELISA wash buffer.
Refolding Protocol
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Charles P. Neff, Mile Cikara, Brian J. Geiss, G. Thomas Caltagirone, Albert Liao, Shaikh M. Atif, Bradley Macdonald, Richard Schaden.
Nucleocapsid protein binding DNA aptamers for detection of SARS-COV-2, Current Research in Biotechnology, Volume 5. 2023. 100132. ISSN 2590-2628. https://doi.org/10.1016/j.crbiot.2023.100132.
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