Ap 2 Aptamer Details
ID# 9373
Structure
Chemistry
DNA
Target
SARS-COV-2 Nucleocapsid
Category
Small Organic
Affinity (Kd)
Aptamers are heated to 95 degrees Celsius for 5 minutes and placed on ice.
Length
73
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dCpdGpdApdGpdGpdCpdTpdCpdTpdCpdGpdGpdGpdApdCpdGpdApdCpdApdApdApdGpdCpdApdGpdCpdGpdApdTpdTpdCpdTpdCpdTpdCpdApdCpdApdGpdApdGpdGpdTpdApdTpdTpdTpdTpdGpdTpdCpdGpdTpdCpdCpdCpdGpdCpdCpdTpdTpdTpdApdGpdGpdApdTpdTpdTpdApdCpdApdGp-3'
Molecular Weight: 22478.6 g/mole
Extinction Coefficient: 691500
Binding Conditions / Buffer
23°C
Binding Temp: Nucleocapsid proteins are incubated 100 ul of aptamers at a 200 nM concentration for 2 hours and then washed with 300 ul of 1x ELISA buffer.
Refolding Protocol
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Charles P. Neff, Mile Cikara, Brian J. Geiss, G. Thomas Caltagirone, Albert Liao, Shaikh M. Atif, Bradley Macdonald, Richard Schaden.
Nucleocapsid protein binding DNA aptamers for detection of SARS-COV-2, Current Research in Biotechnology, Volume 5. 2023. 100132. ISSN 2590-2628. https://doi.org/10.1016/j.crbiot.2023.100132.
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