Aptagen

Antibac1 Aptamer Details

ID# 7976
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Structure
Antibac1 structure
Chemistry
DNA
Target
peptidoglycan
Category
Other
Affinity (Kd)
268.5 nM
Length
55
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-dTpdCpdGpdCpdGpdCpdGpdApdGpdTpdCpdGpdTpdCpdTpdGpdGpdGpdGpdApdCpdApdGpdGpdGpdApdGpdTpdGpdCpdGpdCpdTpdGpdCpdTpdCpdCpdCpdCpdCpdCpdGpdCpdApdTpdCpdGpdTpdCpdCpdTpdCpdCpdCp-3'
Molecular Weight: 16833.83
Extinction Coefficient: 486900

Binding Conditions / Buffer

Selection buffer (PBS containing 1.4 mM MgCl2 and 0.05% BSA); Room temperature (45 mins)
Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

A Graziani et al. High efficiency binding aptamers for a wide range of sepsis 1 bacterial agents. JMB Papers in Press. 2017: JMB16-11004.
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