Anti-NF-kB p65 (R1) Aptamer Details

ID# 7752

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Structure

Chemistry

RNA

Target

NF-kB p65

Sequence (3-char table code)

G A C U/T

5'-rGprAprAprGprCprUprUprAprCprAprAprGprAprAprGprGprAprCprAprGprCprAprCprGprAprAprUprAprAprAprAprCprCprUprGprCprGprUprAprAprAprUprCprCprGprCprCprCprCprAprUprUprUprGprUprGprUprAprAprGprGprGprUprAprGprUprGprGprGprUprCprGprAprAprUprUprCprCprGprCprUprCprAp-3'

Molecular Weight: 26750.22

Extinction Coefficient: 837500

Binding Conditions / Buffer

Selection buffer: 10mM HEPES at pH 7.5, 100mM NaCl, 1mM DTT

Binding Temp: 37°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Wurster, S. E, and Maher, L. J. "Selection and characterization of anti-NF-kB p65 aptamers." RNA, 14(2008): 1037-1047.

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