Aptagen

Anti-human platelet antigen antibodies (Trx-HPA-1a) Aptamer Details

ID# 7786
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Structure
Anti-human platelet antigen antibodies (Trx-HPA-1a) structure
Chemistry
Peptide
Target
Anti-human platelet antigen antibodies (HPA-1a)
Category
Protein
Affinity (Kd)
N/A nM
Length
133
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-MetMetSerAspLys IleIleHisLeuThr AspAspSerPheAsp ThrAspValLeuLys AlaAspGlyAlaIle LeuValAspPheTrp AlaGluTrpCysGly ProValValAlaGly AspAspProArgGlu AspThrTrpGlyPro CysLysMetIleAla ProIleLeuAspGlu IleAlaAspGluTyr GlnGlyLysLeuThr ValAlaLysLeuAsn IleAspGlnAsnPro GlyThrAlaProLys TyrGlyIleArgGly IleProThrLeuLeu LeuPheLysAsnGly GluValAlaAlaThr LysValGlyAlaLeu SerLysGlyGlnLeu LysGluPheLeuAsp AlaAsnLeuValAsp LeuGlnHisHisHis HisHisHis-3'
Molecular Weight: 16956.67
Extinction Coefficient:

Binding Conditions / Buffer

Buffer for ELISA assay: PBS.
Binding Temp: 20-25°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

J Thibaut et al. A novel assay for the detection of anti-human platelet antigen antibodies (HPA-1a) based on peptide aptamer technology. haematologica 97(2012): 696-704.
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