Anti-His Tag (6H7) Aptamer Details
ID# 7564
Structure
Chemistry
DNA
Target
6X His Tag
Category
Protein
Affinity (Kd)
4.6 nM
Length
40
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dGpdCpdTpdApdTpdGpdGpdGpdTpdGpdGpdTpdCpdTpdGpdGpdTpdTpdGpdGpdGpdApdTpdTpdGpdGpdCpdCpdCpdCpdGpdGpdGpdApdGpdCpdTpdGpdGpdCp-3'
Molecular Weight: 12488.1
Extinction Coefficient: 370000
Binding Conditions / Buffer
Selection Buffer (50mM K2HPO4, 150mM NaCl, 0.05% Tween 20 (pH 7.5))
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Kokpinar, et al. "Aptamer-Based Downstream Processing of His-Tagged Proteins Utilizing Magnetic Beads." Biotechnology and Bioengineering, 108(2011):2371-2379.
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