Anti-FLAg M2 Antibody Aptamer (ABA) Aptamer Details
ID# 7715
Structure
Chemistry
DNA
Target
Anti-FLAG M2 Antibody
Category
Protein
Affinity (Kd)
80 nM
Length
30
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dTpdCpdGpdApdTpdTpdTpdCpdCpdTpdTpdApdGpdTpdTpdGpdTpdCpdTpdTpdCpdCpdTpdTp dApdGpdTpdGpdApdGp-3'
Molecular Weight: 9160.02
Extinction Coefficient: 274800
Binding Conditions / Buffer
4% glycerol, 1 mM MgCL2, 1 mM DTT, 50 mM NaCl, 10 mM Tris-HCl, pH 7.5, r.t.
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
A Lakamp and M Ouellette. A ssDNA aptamer that blocks the function of the anti-FLAG M2 antibody. J. Nucleic Acids 2011: doi:10.4061/2011/720798
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