AMP18 Aptamer Details
ID# 8232
Structure
Chemistry
DNA
Target
Ampcillin
Category
Small Organic
Affinity (Kd)
9.8 nM
Length
19
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dTpdTpdApdGpdTpdTpdGpdGpdGpdGpdTpdTpdCpdApdGpdTpdTpdGpdGp-3'
Molecular Weight:
Extinction Coefficient:
Binding Conditions / Buffer
The ssDNA library (500 pmol) was pre-incubated for 3 min at 90 °C and immediately cooled for 1 h at 4 °C in 100 μL of binding buffer (20 mM Tris–HCl, 50 mM NaCl, 5 mM KCl, 5 mM MgCl2, pH 8.0) to stabilize the naturally occurring tertiary structure.
Binding Temp: 4°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Song KM, Jeong E, Jeon W, Cho M, Ban C. Aptasensor for ampicillin using gold nanoparticle based dual fluorescence-colorimetric methods. Anal Bioanal Chem. 2012 Feb;402(6):2153-61. doi: 10.1007/s00216-011-5662-3. Epub 2012 Jan 7. PMID: 22222912.
https://pubmed.ncbi.nlm.nih.gov/28917799/
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