Aptagen

Alpha-fetoprotein (AFP-specific) Aptamer Details

ID# 10111
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Structure
Alpha-fetoprotein (AFP-specific) structure
Chemistry
DNA
Target
Alpha-fetoprotein (AFP)
Category
Protein
Affinity (Kd)
2.37 nM
Length
72 Base Pairs
Aptazyme
No

Sequence (3-char table code)

G A C U/T
5'-dGpdGpdCpdApdGpdGpdApdApdGpdApdCpdApdApdApdCpdApdApdGpdCpdTpdTpdGpdGpdCpdGpdGpdCpdGpdGpdGpdApdApdGpdGpdTpdGpdTpdTpdTpdApdApdApdTpdTpdCpdCpdCpdGpdGpdGpdTpdCpdTpdGpdCpdGpdTpdGpdGpdTpdCpdTpdGpdTpdGpdGpdTpdGpdCpdTpdGpdTp-3'
Molecular Weight: 22471.5 g/mole
Extinction Coefficient: 691100

Binding Conditions / Buffer

20 uL of 1% Bovine Serum Albumin (BSA) buffer
Binding Temp: 25 °C°C

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Chao-Jyun Huang, Hsin-I. Lin, Shu-Chu Shiesh, Gwo-Bin Lee, An integrated microfluidic system for rapid screening of alpha-fetoprotein-specific aptamers, Biosensors and Bioelectronics, Volume 35, Issue 1, 2012, Pages 50-55, ISSN 0956-5663, https://doi.org/10.1016/j.bios.2012.02.024.
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