AHD50-1 against 1-aminohydantoin Aptamer Details

ID# 8762

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Structure

Chemistry

DNA

Target

1-aminohydantoin (AHD)

Sequence (3-char table code)

G A C U/T

dCpdTpdCpdGpdTpdTpdApdTpdGpdApdCpdTpdApdApdCpdGpdCpdTpdApdGpdCpdCpdCpdGpdGpdGpdGp

Molecular Weight:

Extinction Coefficient:

Binding Conditions / Buffer

Binding buffer: pH 7.4. 100mM NaCl, 2mM MgCl2, 20mM Tris-HCl, 1 mM CaCl2, 5mM KCl. 25°C in the dark for 2 hours.

Binding Temp: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Zheng, X., Jiang, S., Ren, Y., Wang, S., Xie, Y., & Le, T. (2024). High-efficient selection of aptamers by magnetic cross-linking precipitation and development of aptasensor for 1-aminohydantoin detection. LWT - Food Science and Technology, 199. https://doi.org/10.1016/j.lwt.2024.116128

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