Aflatoxin M1 Aptamer (AFAS3) Aptamer Details
ID# 7889
Structure
Chemistry
DNA
Target
Aflatoxin M1
Category
Small Organic
Affinity (Kd)
35.6 nM
Length
73
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdTpdCpdCpdGpdTpdCpdApdCpdApdCpdCpdTpdGpdCpdTpdCpdTpdGpdApdCpdGpdCpdTpdGpdGpdGpdGpdTpdCpdGpdApdCpdCpdCpdGpdGpdApdGpdApdApdApdTpdCpdGpdCpdApdTpdTpdCpdCpdCpdCpdTpdGpdTpdGpdGpdTpdGpdTpdTpdGpdGpdCpdTpdCpdCpdCpdGpdTpdApdTp-3'
Molecular Weight: 22383.47
Extinction Coefficient: 664700
Binding Conditions / Buffer
20 mM Tris-HCL, 100 mM NaCl, 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Malhotra, S., Pandey, A., Rajput, S. and Sharma, R. "Selection of aptamers for Aflatoxin M1 and their characterization." Journal of Molecular Recognition 27 (2014): 493-500.
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