ADP Aptamer Details

ID# 7653

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Structure

Chemistry

RNA

Target

ADP

Sequence (3-char table code)

G A C U/T

5'-rGprGprGprCprGprAprCprCprCprUprGprAprUprGprAprGprCprAprCprAprCprGprAprGprGprGprGprGprAprAprAprCprCprCprCprGprGprAprCprAprAprUprCprAprGprAprCprAprCprGprGprUprGprUprUprCprGprAprAprAprCprGprGprUprGprAprAprAprGprCprCprGprUprAprGprGprUprUprGprCprCprCprUprUprUp-3'

Molecular Weight: 27540.71

Extinction Coefficient: 837700

Binding Conditions / Buffer

Selection Buffer ((50 mM HEPES [pH 7.4], 25 mM MgCl2, 150 mM NaCl) containing 10 g/ml tRNA)

Binding Temp:

Refolding Protocol

If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference

Srinivasan et al. "ADP-Specific Sensors Enable Universal Assay of Protein Kinase Activity." Chemistry & Biology, 11(2004): 499-508.

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