Adipose Tissue (adipo-8) Aptamer Details
ID# 7708
Structure
Chemistry
DNA
Target
Mature 3T3-L1 adipocytes (adipose tissue)
Category
Cells
Affinity (Kd)
17.8 nM
Length
87
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdTpdGpdApdGpdApdApdGpdCpdGpdTpdCpdGpdGpdTpdGpdTpdGpdGpdTpdTpdApdApdAp dCpdApdCpdGpdGpdApdApdCpdGpdApdApdGpdGpdTpdGpdCpdApdGpdGpdApdApdGpdApdTpdTpdTpdGpdTpdCpdGpdApdTpdGpdCpdGpdGpdTpdGpdCpdCpdTpdGpdApdGpdCpdGpdGpdGpdCpdTpdGpdGpdCpdApdApdGpdGpdCpdGpdCpdApdTpdAp-3'
Molecular Weight: 27226.7
Extinction Coefficient: 862800
Binding Conditions / Buffer
4.5 mg/ml glucose and 5 mM MgCl2 in D-PBS with calcium chloride and magnesium chloride (Sigma), yeast tRNA (0.1 mg/ml) and BSA (1 mg/ml). pH 7.1-7.5. 4°C
Binding Temp:
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
J. Liu et. al. "Selection of aptamers specific for adipose tissue." PLoS One, 7(2012): e37789.
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