Adenine (12E4) Aptamer Details
ID# 7507
Structure
Chemistry
RNA
Target
Adenine
Category
Small Organic
Affinity (Kd)
10 µM
Length
91
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-rGprGprGprAprGprAprGprGprAprUprAprCprUprAprCprAprCprGprUprGprAprUprAprGprGprAprCprGprAprUprUprAprUprCprGprAprAprAprAprUprCprAprCprCprAprGprAprUprUprGprGprAprCprCprCprUprGprGprUprUprAprAprCprGprAprUprCprCprAprUprUprGprCprAprUprGprUprAprGprCprAprGprAprAprGprCprUprUprCprCprGp-3'
Molecular Weight: 29362.78
Extinction Coefficient: 927600
Binding Conditions / Buffer
Binding Buffer: 100 mM HEPES, 500 mM NaCl, 5 mM MgCl2 (pH 7.3)
Binding Temp: RT°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Meli, M., et al. "Adenine-aptamer complexes: a bipartite RNA site that binds the adenine nucleic base." Journal of Biological Chemistry, 227 (2001): 2104-2111.
Request a Quote
Have your aptamer oligo synthesized by Aptagen.
