Ade1301b Aptamer Details
ID# 9629
Structure
Chemistry
DNA
Target
Adenosine & ATP
Category
Small Organic
Affinity (Kd)
0.2 (Ade) & 2.5 (ATP) µM
Length
40
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdCpdGpdApdCpdGpdTpdTpdTpdGpdCpdGpdApdTpdGpdApdGpdApdApdApdCpdGpdTpdApdTpdGpdGpdTpdTpdTpdCpdGpdApdApdGpdGpdTpdCpdGpdTp-3'
Molecular Weight: 12431.1 g/mole
Extinction Coefficient: 396700
Binding Conditions / Buffer
SELEX buffer: 50 mM Tris, pH 7.6, 500 mM NaCl, 20 mM MgCl2
Binding Temp: 25°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
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