Acute myeloid leukemia cells (KH1C12) Aptamer Details
ID# 7483
Structure
Chemistry
DNA
Target
Acute myeloid leukemia cells (HL60)
Category
Cells
Affinity (Kd)
4.5 nM
Length
68
Aptazyme
No
Sequence (3-char table code)
G
A
C
U/T
5'-dApdTpdCpdCpdApdGpdApdGpdTpdGpdApdCpdGpdCpdApdGpdCpdApdTpdGpdCpdCpdCpdTpdApdGpdTpdTpdApdCpdTpdApdCpdTpdApdCpdTpdCpdTpdTpdTpdTpdTpdApdGpdCpdApdApdApdCpdTpdGpdGpdApdCpdApdCpdGpdGpdTpdGpdGpdCpdTpdTpdApdGpdTp-3'
Molecular Weight: 20896.62
Extinction Coefficient: 647700
Binding Conditions / Buffer
Binding Buffer (4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/ml tRNA and 1mg/ml BSA, dissolved in Dulbecco's PBS with MgCl2 and CaCl2) containing 20% FBS.
Binding Temp: 4°C
Refolding Protocol
If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Reference
Sefah, Kwame, et al. "Molecular recognition of acute myeloid leukemia using aptamers." Leukemia, 23 (2009):235-244
Request a Quote
Have your aptamer oligo synthesized by Aptagen.
